Preparation, Characterization and Cytotoxicity Evaluation of StarchBased on Electrospun Nanofibrous Scaffold Incorporated with Bioactive Compound from Mollugo Nudicaulis

Abstract

This research manuscript details the fabrication and characterization of starch-based 
electrospun nanofibrous scaffolds enriched with 12-(10-carboxydecanoyloxy)-12-
oxododecanoic acid (Compound-1), sourced from the ethanolic extract of Mollugo 
nudicaulis. Varying concentrations of Compound-1 (0%, 20%, 30%, and 40% v/v) were 
incorporated into starch solutions, which were subsequently transformed into nanofibers 
using electrospinning. The resulting nanofibers were comprehensively analyzed for 
morphology, size distribution, thermal stability, FT-IR spectra, and cytotoxicity potential. 
The nanofibers loaded with Compound-1 exhibited consistent and uniform morphology, 
with average diameters falling within the range of 76 to 98 nm. Remarkably, the 
incorporation of Compound-1 led to an improvement in the thermal stability of the 
nanofibers when compared to the unencapsulated Compound-1. FT-IR analysis further 
confirmed interactions between the starch matrix and Compound-1. Most notably, 
nanofibers loaded with 40% Compound-1 demonstrated significantly enhanced viability 
in HaCat cells. These findings suggest that the developed starch-based electrospun 
nanofibrous scaffolds, enriched with Compound-1 from Mollugo nudicaulis, possess 
promising potential as bioactive nanomaterials for various biomedical applications. 
However, it is essential to conduct further in vitro and in vivo studies to validate these 
initial observations.