Low Density Poly Ethylene Degradation (LDPE) Degradation Using Soil Bacteria from Plastic Dumped Sites

Abstract

Low density polyethylene (LDPE) is one of the dominate usage of plastics in all applications which also leads to the plastic pollution. Biodegradation was an unharmed as well as tedious process. On this study, plastics can be able to degrade by competent microorganism by isolated the microbes from garbage area assuming that the potent strain can be accessible. The degrading bacteria can be isolated by enrichment method where the artificial media was prepared with LDPE strips as a sole carbon source. The isolated three strains EPSP1, EPSP2, EPSP3 were used for degrading studies. The bacterial biomass estimated of these strains done by the protein concentration which estimated EPSP1, EPSP2, EPSP3 has to be 0.18, 0.24, 0.2 µg /ml respectively. Biodegradation of LDPE can be observed by the weight loss of the LDPE films. Higher degradation occurs in EPSP2 strain (9.6%) which showed highest weight reduction. The potent strain EPSP2 undergoes for 16SrRNA sequencing which reveals that it was a Bacillus sp i.e. Priestia aryabhattai. Hence, this work paved that LDPE can be degraded by garbage sited microorganism and used for the further studies to determine the plastic pollution problems.