Developing and Characterizing an Improved Antihyperlipidemic Nanosuspension of Tagetes erecta

Abstract

Tagetes erecta nanosuspension was tested for anti-hyperlipidemic and antioxidant effects, as well as increased solubility and permeability with curcumin. The nanosuspension was designed using a sonication process and Tagetes erecta (NS-TE) was extracted by methanolic extract and Curcumin (derived from Curcuma longa rhizomes). According to literature review report, the extracts were collected from Tagetes erecta flower (NS-TE). Tagetes erecta flower extract with curcumin (NS-CTE) were produced and tested by SEM, UV spectroscopy, and FTIR particle size, entrapment efficiency, zeta potential. The particle size of NS-TE and NS-CTE were determined to be 138.3 nm and 100.9 nm and entrapment efficiency of 56.32 (NS-TE) and 89.6 (NS-CTE) respectively. According to zeta potential study NS-TE and NS-CTE had potentials of -17mV and -18.9mV, respectively. SEM analysis of NS-TE and NS-CTE revealed the particle has produced spherical shape and smooth surface. The λ maximum for NS-TE was determined to be 250 nm, and the calibration curve revealed a linear curve, indicating a response that follows Beer Lamberts law. The presence of functional groups such as amide, amines, aldehyde, nitro compounds, and carboxylic acids was revealed by FTIR spectroscopy. NS-TE and NS-CTE were tested for antioxidant activity in vitro using nitric acid and hydroxyl radical scavenging assays, as well as antihyperlipidemic activity in vivo using PTU-induced hyperlipidemia. At a dose of 100 mg/kg bd. wt., NS-TE and NS-CTE demonstrated considerable antioxidant and anti-hyperlipidemic efficacy.