Network Pharmacology of Pareesha (Thespesia Populnea. L) and Shirisha (Albezia Lebbeck. L) in the Management of Diabetic Wound. an In-Silico Approach towards Concept of Pratinidhi Dravya

Abstract

Introduction: The concept of Pratinidhi Dravya mentioned in classics, an alternative drug that can be used as the substitute of another when the original is not available Geographically or no longer in use or is difficult to obtain. Shirisha is considered as Pratinidhi Dravya for Pareesha, where both drugs possess wound healing activity. Ayurveda maintains its comprehensive approach to healing while adapting to altering resource availability by using this concept.

Objectives: To compare Pareesha and shirisha, through network pharmacology and prove its relevance as pratinidhi Dravya in today’s era.

Methods: Pareesha and Shirisha phytochemicals were sourced from Dr. Dukes, IMPPAT databases, previous studies. Using Lipinski’s criteria, the drug-likeness of phytochemicals associated with PubChem CID was examined using Swiss ADME. Target was predicted by Binding DB and Swiss target database Uniprot used to acquire Gene ID. KEGG pathways were analysed using the String database, networks framed using Cytoscape 3.7.2.

Results: It discovered from bioinformatics the key phytochemicals identified include Kaempferol, Quercetin, Gossypol found in Pareesha, Acacetin, Okanin, and Echinocystic acid in Shirisha have been shown to interact significantly with proteins like MAPK3, PRKCA, PRKCG, of Pareesha and PTP1B, CA2, and FABP3 of Shirisha regulating sphingolipid signalling pathway, serotonergic synapses, inflammatory mediator control of TRP channels, and EGFR tyrosine kinase inhibitor resistance that are involved in diabetic wound.

Conclusions: The in-silico method is a useful technique for finding multi-compound treatment plans, improving drug discovery, and providing scientific validation to classical writings by proving that the concept of Pratinidhi dravya remains valid even today.