Silver Nanoparticles Inhibition of SAP 7 and SAP 9 Gene Expression in Candida albicans Isiolates from Diabetic Foot Ulcers

Abstract

Candida albicans is the most common yeast species in diabetic foot patients who are at risk of growing diabetic foot ulcers, this has something to do with fungal infections. This study was conducted on 203 clinical samples of diabetic patients with diabetic foot ulcers  for the period from January 15 to May 15, 2022. All samples were grown on Sabouraud Dextrose Agar (SDA) and incubated at 37°C for 24–48 hours under aerobic condition . Identification of Candida albicans isolates depends on morphological characteristics in culture medium, germ tube formation, chlamydospore formation, and CHROMagar  and using the Vitek 2 compact system . It was found (15) isolates from C.albicans .  The results indicated the presence of SAP7,SAP9 genes in all isolates (100%; n=15) of C.albicans.The treated of 4 isolates with AgNPs , our results show an increase in the gene expression values of SAP 7 for the two isolates CAA1 and CAA11 and tow isolates, CAA2 and CAA5 had reduced gene expression values .The results of our study on SAP 9 gene expression after treatment with AgNPs were that there was an increase in the gene expression values of the two isolates CAA1 and CAA11 and gene expression values decrease for two isolates, CAA2 and CAA5.