A Histopathological Spectrum of Hepatic Lesions in Correlation with Clinical Findings

Abstract

INTRODUCTION- Liver is affected by a wide spectrum of various primary and secondary diseases. Liver diseases can be diagnosed by clinical, biochemical and radiological examinations. However, liver biopsy plays a crucial role in diagnosis and management of liver diseases.

AIM-   To determine the prevalence and patterns of liver lesions in our hospital.

OBJECTIVE-   To study the histopathological findings in various liver lesions and to correlate with the clinical findings.

STUDY DESIGN- A hospital based cross-sectional study.

STUDY PERIOD- Three years from Feb 2022 to Feb 2024

SAMPLE SIZE- Approximate sample size is twenty.

MATERIALS AND METHODS- This was a cross-sectional study conducted in department of pathology, Asram medical college, Eluru . The study duration was three years from February 2022 to February 2024. The Age, gender, clinical and radiological findings along with liver function tests results were recorded in all the cases. Formalin fixed liver biopsy tissues were processed routinely. These tissues were dehydrated in ascending grades of alcohol, cleared with xylene and embedded in paraffin and   blocks were prepared. The blocks were then cut in 2 to 5 micrometer thickness using microtome. These sections were stained with Haematoxylin & Eosin. Some special stains like Reticulin, Masson’s trichrome (MT), Periodic acid Schiff stain(PAS) and Prussian blue (Perl’s) were used in selected cases. The findings were recorded and analyzed.

INCLUSION CRITERIA- All the adequate liver biopsies received in the department of pathology.

EXCLUSION CRITERIA- Inadequate liver biopsies were excluded from the study.

STASTICAL ANALYSIS-   Data was entered in excel and analyzed with spss18. Data was presented with proportions, tables and graphs. Chisquare test was applied for categorical variables and P value of 0.05 was considered statiscally significant.