Invitro Antioxidant Studies and Characterisation of Anthocyanin using UV, FTIR and UPLC in Nerium Oleander Flowers, Collected as Temple Floral Waste.

Abstract

The present study has been focused on the investigation of the phytochemical characteristics and antioxidant properties of  Nerium oleander flowers, collected as temple floral waste. Preliminary phytochemical analysis of the methanolic flower extract revealed the presence of diverse components including alkaloids, flavonoids, phenolic compounds, tannins, terpenoids, and anthocyanin. UV-Vis and IR spectroscopic analyses confirmed the presence of key compounds such as anthocyanins, flavonoids, polyphenols, and tannins . The presence of anthocyanins was conclusively confirmed through Ultra-Performance Liquid Chromatography (UPLC), providing robust evidence of their occurrence in the extract.The methanolic extraction was able to scavenge free radicals as tested through DPPH radical scavenging assay.The use of temple floral waste as a source material highlights the study's focus on sustainable resource utilization.