Synthesis, Characterization and its Biological Activity of Novel Bis (Biphenylsulfonylacetamido) Oxacalix[4] Arene Derived from Oxacalixarene

Abstract

Introduction: Supramolecular chemistry involves the study of non-covalent interactions between molecules, enabling the design of complex molecular systems with tailored properties. These interactions, such as hydrogen bonding, electrostatic forces, π-π stacking, and van der Waals forces, allow the formation of supramolecular compounds with unique structural and functional characteristics. These compounds are increasingly being explored for their potential applications in biological systems, as they can mimic natural biomolecular interactions. The dynamic nature of supramolecular assemblies provides opportunities for the development of compounds with enhanced selectivity and efficiency in targeting biological macromolecules, such as enzymes, receptors, and nucleic acids. This research investigates novel supramolecular compounds designed to interact with specific biological targets, offering promising strategies for drug discovery and therapeutic interventions in various diseases.

Objective: This study focused on designing of Oxacalix[4]arene based novel compound and evaluating its anti-tuberculosis ,anti-oxidant and anti-cancer activity.

Methods:  Conventional reaction method was used for the synthesis of Bis (biphenylsulfonylacetamido) Oxacalix[4] arene

Compound and H¹ NMR , ESI-MS and IR Spectroscopy was used for the characterization of compound. Anti-tuberculosis activity was performed using Zone Inhibition Method. Anti- oxidant study was assessed using DPPH technique. In-vitro cytotoxicity was assessed on the Hella cell line using the MTT assay.

Result: H¹ NMR , ESI-MS and IR Spectroscopy confers the synthesis of Bis (biphenylsulfonylacetamido) Oxacalixarene

Compound. In vitro assessments on Hella cells demonstrated a concentration-dependent reduction in cell   survival, yielding an IC₅₀ of 358.3 µg/ml. When the compound was tested for anti-oxidant activity it found to be active compared to the standard ascorbic acid. The largest zone of inhibition (ZI) produced by the test compound was compared to that of the positive control to determine its antibacterial efficacy against M. tuberculae.

Conclusion: The result highlights the successful synthesis of novel compound and its efficacy towards anti-cancer , anti-oxidant and anti-tuberculosis activity , making it an intriguing topic for upcoming research on drug development.