Preparation, Characterization and Qualitative Assessment of Electrospun Cranberry Loaded Nanofiber Membrane- In Vitro Study

Abstract

Introduction: Periodontal disease is a long-term inflammatory condition that leads to the gradual deterioration of the tissues supporting the teeth, potentially leading to tooth loss. While mechanical debridement is standard, combining it with antimicrobial therapy improves outcomes. Cranberry’s anti-inflammatory and antimicrobial properties show promise in periodontal care. Electrospinning creates nanofiber membranes for localized delivery, enabling controlled release.

Objectives: This study develops and analyzes cranberry-infused electrospun nanofiber membranes for periodontal therapy.

Methods: A nanofiber membrane was developed by blending 50 mg of cranberry powder with 15% polyvinyl alcohol (PVA) and 2% chitosan in a 4:1 ratio, followed by overnight stirring. The mixture was electrospun using an 18G blunt needle at 15.9 kV, with fibers collected 12 cm away at a 0.005 ml/min flow rate. Morphology of nanofiber was analysed through SEM, further characterization was done by analysing drug release pattern, degradation rate, antimicrobial activity.

Results: The cranberry-loaded nanofiber membrane exhibited uniform distribution, no bead formation, an average diameter of 466 ± 12 nm, and 75% degradation by day 15. It showed controlled drug release with an initial burst followed by sustained release and strong antimicrobial properties, inhibiting bacterial growth by over 99.9995%, making it a promising option for periodontal therapy.

Conclusions: In conclusion, the cranberry-loaded nanofiber membrane exhibited uniform distribution, controlled drug release, and antimicrobial activity, making it a promising option for periodontal therapy. Its properties suggest effectiveness in periodontitis treatment, providing sustained benefits and improved outcomes.