Studying Protective Role of Nano-Extract of Fruits of Cardi Myxa Plant Against Effects of Indomethacin Drug on Kidneys of Male White Rats.

Abstract

The current study was conducted in the animal house/college of science/University of Kufa, This study aimed to reveal the protective role of the nano-extract of the fruits of the Cardi myxa  plant in the histological structure of the kidneys of male albino rats treated with indomethacin, (40) male rats were used , and the ages of the male rats ranged between (8-10) weeks and their weights ranged between (200-250) g, the animals were divided into (8) groups, each group included (5) male rats, the first group represented the control group and the second group  was treated with indomethacin drug with a concentration of (0.143) mg/kg, the third group is the ZnO group with a concentration of (1) mg/kg,and the fourth and fifth groups that are treated with the nano-extract group with two concentrations of (20 and 30 mg/kg) respectively, while  the sixth and seventh groups are treated with the nano-extract with two concentrations of (20 and 30 mg/kg) + indomethacin drug respectively which was treated with the drug half an hour after dosing with the nano-extract, and the eighth group was dosed with the aqueous extract of Cardi myxa plant with a concentration of (500) mg/kg + indomethacin drug with a concentration of (0.143) mg/kg for the period (40 days,fter that the animals were sacrificed and the histological sections of the kidney tissue were made. The results of the histological sections of the kidney of white rats showed that the control group had normal tissue for the kidney, as for the indomethacin drug group,the results showed that shrinkage of the glomerulus, widening of the Bowman's space  and necrosis in the renal tubules, also the results of the ZnO group revealed the effect of Zno on the kidney tissue such as complete renal glomeruli retraction, necrosis of the urenal tubules with infiltration of inflammatory cells, as for the results of the fourth and fifth groups which were treated with  the nano-extract groups with two concentrations (20 and 30) mg / kg, the results showed that the histological structure of the kidney was close to normal and the appearance of the epithelial cells of the renal tubules with the normal shape compared to a control group, As for the results of the sixth and seventh groups, the two groups of nano-extract (20 and 30 mg / kg) + indomethacin drug, their results showed a slight effect on the cells lining the renal tubules and the appearance of the normal histological structure of the kidney, and the results of the eighth group which was treated with the plant aqueous extract group with concentration (500 mg / kg) + indomethacin drug at a concentration of (0.143 mg / kg ), as the results showed little necrosis in the lining cells of renal tubules with moderate effects of renal glomeruli.