Nephroprotective Property of Ethanolic Extract of Moringa Oleifera Bark Against Gentamicin Induced Nephrotoxicity in Wistar Rats

Abstract

Gentamicin is reported to cause acute renal failure in 20% of hospitalized patients due to oxidative stress on the proximal tubule of kidneys. Moringa oleifera (MO) bark is reported to contain a high concentration of antioxidants. Hence this study aimed to evaluate the nephroprotective effect of Moringa oleifera bark against gentamicin-induced nephrotoxicity. Ethanolic extract of Moringa oleifera bark was studied for its nephroprotective effect in experimental rat models. Nephrotoxicity was induced by Gentamicin by administering 80mg/kg for 8 days i.p. MO bark extract at doses of 250 mg/kg and 125mg/kg was evaluated for nephro-protective properties. The nephroprotective action was evaluated by blood serum parameters such as Creatine, Urea, Total protein, Uric acid, LDH, and tissue parameters such as MDA, GSH, LPO, and SOD followed by histopathology studies of the kidney. Gentamicin-treated rats exhibited, elevated levels of blood serum creatine, total protein, LDH, uric acid, urea, and LPO, and a decrease in antioxidants such as SOD, catalase, and GSH compared to the control group. Treatment with a high dose (250mg/kg) and low dose (125mg/kg) of extract of Moringa oleifera bark shows a significant decrease in the levels of blood serum creatine, total protein, LDH, uric acid, urea, and LPO and increase in the levels of antioxidants such as SOD, catalase, GSH compared to gentamicin group. In addition, gentamicin led to cellular damages including peritubular congestion, epithelial desquamation, blood vessel congestion, and necrosis, all of which were partially recovered by Moringa oleifera bark extract.