Development and Validation of UV-Visible Spectrophotometric Method for Estimation of Fisetin in Bulk Form

Abstract

Introduction: Fisetin (FS), a flavonoid dietary element, has recently gained increasing attention. It is also known as (2-(3,4-Dihydroxyphenyl)-3,7-dihydroxy-4H-1-benzopyran-4-one) [2]. It is typically found in a variety of fruits and vegetables, including strawberries, onions, apples, and cucumbers.

Objectives: The aim of this work is to provide a straight forward UV-visible spectrophotometric approach for determining Fisetin in pure form and validation of the method. Using double beam UV-visible spectrophotometer and solvent using methanol, method was developed and validated for Fisetin.

Methods: The study focused on generating a specific, linear, accurate and precise UV spectrophotometric method for estimation of Fisetin in Bulk Form. Statistical validation followed International Conference of Harmonization (ICH) specifications. The method's parameters were assessed within a concentration range of 2 to 10 µg/ml, with recovery rates specifying accuracy and low % relative standard deviation (RSD) values confirming precision. Limits of detection (LOD) and quantification (LOQ) for Fisetin were determined.

Results: A coefficient of correlation of 0.998 was discovered in accordance with Beer's law. After determining the technique's sensitivity, the limit of detection and limit of quantification were determined to be 0.457µg/ml and 1.386µg/ml respectively

Conclusions: The developed UV spectrophotometric method proved suitable for the quantitative estimation of Fisetin, offering rapid and accurate analysis. The results underscore the method was linear, consistent, precise, and affordable and LOD, LOQ, area under the curve were within the specified concentration range.