Assessing the Antimicrobial Potential of Probiotics and Probiotic-Derived Supernatants against Enterococcus Faecalis: An in Vitro Analysis

Abstract

Context:The primary goal of endodontics is to eradicate infected pulpal tissues and prevent periapical infections. However, the persistence of Enterococcus faecalis is commonly linked to failed root canal treatments and recurrent periapical infections..

Aim: To evaluate and compare the antibacterial efficacy of Probiotics and Cell free supernatant of Probiotics against E. faecalis.

Settings and Design: The antibacterial efficacy of the Probiotics and Cell free supernatant of Probiotics were assessed and compared in vitro against E. faecalis.

Methods and Material: The minimum inhibitory concentration (MIC) was determined using 96-well culture plates, and the optical density of each well was measured at 600 nm. The agar well diffusion method was performed to measure the zones of inhibition in millimeters.

Statistical analysis used: Data analysis was conducted using a one-way ANOVA test followed by post hoc analysis with Bonferroni correction.

Results: The MIC of probiotics against E. faecalis was optimized at 10 mg/mL. Probiotics and their metabolic by-products showed distinct zones of inhibition, demonstrating their antimicrobial activity against E. faecalis..

Conclusions: The results of this study suggest that probiotics and their cell-free supernatant could be incorporated into root canal medicaments, sealers, and irrigants, given their potent antimicrobial efficacy against E. faecalis.