Formulation and Investigation of Herbal Anti-inflammatory Gel from Hydroalcoholic Leaf Extracts of Cichorium intybus and Sida cordata

Abstract

Herbal remedies remain a valuable source of solutions for various health conditions, including Inflammation. This study focused on developing and evaluating a novel herbal gel formulated with hydroalcoholic extracts of Cichorium intybus and Sida cordata leaves. The research involved the extraction and analysis of the chemical components from the leaves to identify their bioactive constituents. Two gel formulations (F1 and F2) were prepared and assessed for physicochemical properties, including color, texture, spreadability, pH, and viscosity, ensuring stability and usability. The antioxidant activity of the gels was evaluated using DPPH and nitric oxide scavenging methods, demonstrating their free radical-scavenging potential. The antibacterial properties were tested through the disc diffusion method, revealing notable activity against selected bacterial strains. Additionally, the anti-inflammatory potential was assessed using the HRBC membrane stabilization assay, a method to gauge the ability to inhibit inflammation by stabilizing red blood cell membranes under stress.

The results highlighted the significant therapeutic potential of these gels, with promising antioxidant, antibacterial, and anti-inflammatory properties. These findings support the use of Cichorium intybus and Sida cordata extracts as effective agents for inflammation-related conditions, emphasizing their role in advancing herbal formulations for natural and accessible health treatments.