Quantification of Human DNA in Dental Calculus and Blood: A Cross-Sectional, Descriptive Study
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Abstract
Background: Whole blood is the most common sample type used for obtaining high purity DNA but previous studies have reported that DNA is also present in dental calculus.So we investigated human DNA in nonarchaeological dental calculus as well as we compared the amount of human DNA in dental calculus and blood.
Methodology: The present comparative – study was conducted to evaluate the amount of human DNA in dental calculus and blood. This study was conducted in the 35 subject aged 18-60 years reporting to the OPD of Department of Public Health Dentistry, Babu Banarasi das college of Dental Sciences, Lucknow. The samples of dental calculus and blood were collected and underwent DNA analysis.
Results: The mean of concentration of DNA in blood (70.19±14.55µg/ml) was higher thatin dental calculus (54.92± 11.729µg/ml), but the difference in both the means were found to be non-significant (p˂0.218).
Conclusion: Dental calculus can act as a good substitute and replace collection of DNA from human blood and will be successfully used to serve as an investigative tool for forensic purpose.