Formulation of Topical Gel Using Cissus Quadrangularis Plant Extract- An In Vitro Study

Abstract

Plants have been used by humans as a natural source of remedies and cures since ancient times. Medicinal herbs have gained popularity due to their widespread application and low toxicity. Perennial climber C.quadrangularis is widely utilized in Indian traditional medicine systems. It has been shown to have analgesic, antibacterial, antifungal, antioxidant, anthelmintic, and bone fracture healing effects. The analgesic properties of this plant extracts are mostly beneficial for arthritis, and bone repair which helps in improving people's comfort and promoting mobility. The study aimed to assess the anti-inflammatory property of C.quadrangularis. The microbial activity of the C.quadrangularis plant extract was prepared using different solvents such as ethanol, methanol, DMSO, chloroform, ethyl acetate. The antimicrobial activity of the plant extract was analyzed against both gram-positive and gram-negative bacteria namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus,Bacillus subtilis, Klebsiella pneumoniae, Proteus mirabilis by agar well diffusion method. They reveal a significant zone of inhibition against Bacillus subtilis. The preliminary phytochemical screening of the plant extract was performed to identify the compounds such as flavonoids, tannins, terpenoids, saponin, and steroids. The topical formulation was prepared using a methanolic extract of C.quadrangularis. The in vitro study of C.quadrangularis infused topical gel using the HET-CAM method shows that haemorrhage was not observed. The resulting formulated topical gel had antibacterial, antifungal, analgesic and anti-inflammatory effects and it is also safe and eco-friendly.