Gastroprotective Effect of Trichosanthes cucumerina fruit, Hydro-Alcoholic Extract on Experimentally Induced Gastric Ulceration in Albino Wistar Rats.
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Abstract
Objectives: The objective of the present study is scientifically validate the gastro-protective effect and derive the possible mechanistic activity of Trichosanthes cucumerina Linn. fruit, hydro-alcoholic extract against aspirin plus pylorus ligation, ethanol plus HCl induced, acetic acid induced and cold restraint stress induced ulcer models in wistar albino rats.
Methods: The gastroprotective effect of TCHAE (125, 250 & 500 mg/kg, p.o.) was evaluated on both the chemical and physical (Aspirin plus pyloric ligation, ethanol plus HCl, Acetic acid, and Cold restrain stress,) ulcerogens induced ulceration in wistar rats. The mechanistic activity of TCHAE was tested on various gastric-ulcer parameters, namely gastric pH, volume, total and free acidity, output, total protein level, level of mucoprotein and glycoprotein content, antioxidant markers level like LPO, SOD and CAT. Ulcer index and % ulcer protection was assessed to determine the gastroprotective potential.
Results: TCHAE (250-500 mg/kg, p.o.) significantly decreases the ulcer index (p<0.0001) against all the four tested gastric-ulcer models. In the aspirin plus pylorus ligation model exhibited % protection of 67.56 and 74.66, respectively. In ethanol plus HCl induced ulcer model, 250 mg/kg and 500 mg/kg displayed % protection of 56.68 and 70.32, respectively. In acetic acid induced chronic ulcers model 250 mg/kg and 500 mg/kg displayed % protection of 50.60 and 68.83, respectively. Cold restraint stress (CR Stress) induced ulcer model, 250 mg/kg and 500 mg/kg displayed % protection of 63.28 and 73.29, respectively. A decrease in aggressive parameters like acid volume, LPO and an increase in protective parameters like total carbohydrate and total protein ratio, SOD, CAT were observed during the estimation of biochemical parameters justifying its protective effects.
Conclusions: The present study indicates that the Trichosanthes cucumerina Linn. fruit, hydroalcoholic extract possesses significant gastroprotective property through the defensive mechanism.