Genotypic Detection of HPV 16 and HPV 18 in Cases of Cervical Cancer from Southern Odisha, India

Abstract

Introduction: Human Papilloma virus (HPV) is one of the major risk factors for the occurrence of cervical cancer (CaCx) globally including India. The prevalence of HPV among the patients with CaCx is found to be varied.

Objectives: This study was carried out with an objective to find out the prevalence of HPV 16 and HPV 18 in CaCx samples in southern Odisha, India.

Methods: Cervical tissues samples were collected and subjected to histopathological analysis. Histopathologically positive samples were further evaluated for HPV infection by PCR method with little modifications with an initial heat treatment during DNA isolation. Two subtypes of HPV (HPV 16 and HPV 18) were investigated in this study. Description analysis was done for the presentation of generated data.

Results: A total of 122 positive cases, confirmed by histopathology for CaCx were considered for DNA isolation followed by HPV 16 and HPV 18 subtype identification. Tissue samples with a lysis step of 2-4 hours incubation at 100˚C were significantly resulted with good yield of DNA. About 98 cases (80.32%) were found to be positive for HPV infection including HPV16 in 80 (81.63%) cases, HPV18 in 16 cases (16.33%) and both HPV 16 and HPV 18 in two cases (2.04%).

Conclusions: The prevalence of HPV infection was found to be high in women diagnosed with CaCx in southern Odisha, which is a warning alert for the early HPV vaccination as well as early diagnosis of HPV infection.