Restoration of Intestinal MCP-1 and Protein Oxidation Products by Spondias pinnata Bark Extract in Etoposide Induced Rat Gut Mucositis

Abstract

Introduction Chemotherapeutic agents, despite their anticancer properties, can cause mucositis by damaging normal cells. Protecting these cells during therapy is crucial.

Objectives: Present study aims to assess the impact of Spondias pinnata (SP) bark extract on Monocyte chemoattractant protein-1, Cycloxygenase-2, Altered Oxidative protein products, and Superoxide Dismutase levels in an etoposide-induced mucositis rat model, comparing it with normal control and etoposide control groups. This evaluation is essential for understanding the protective effects of SP extract during chemotherapy.

Methods: 78 Albino Wister rats were categorized into 13 groups including normal control group, etoposide control, SP control, etoposide treated with SP bark extract groups, each of these groups were sub-divided based on daily treatment (24, 48, 72 & 96 hr). Mucositis was induced by single dose of intra-peritoneal injection of Etoposide (45mg/Kg body wt.). Time of injection of etoposide was considered as zero hr and the parameters were studied by sacrificing the animals on daily basis, until 96hr. SP dose of 200mg/Kg body wt. was given to SP control and treated group.

Results: SP bark extract led to moderate upregulation of Monocyte chemoattractant protein-1 (p=0.019) and Superoxide Dismutase (p=0.0000542) levels at 96 hr. Additionally, Altered Oxidative protein products (p=0.0381) and Cycloxygenase-2 levels decreased significantly.

Conclusions: These finding looks promising in alleviating the metabolic imbalance due to etoposide and SP bark extract can be used as an adjuvant during chemotherapy to protect the normal cells and could help them improve their quality of life.