Molecular Detection of the Intracellular Adhesion Genes in Clinical Isolates of Staphylococcus Aureus

Abstract

One hundred ninety (190) clinical samples were collected from different sources (wounds, burns, urine and blood) from Baquba Teaching Hospital, the Consulting Clinic and in Diyala Governorate for the period from September 15/ 2022 to December 15/ 2022. Fifty 50 (26%) S. aureus isolates screened by phenotypic and Biochemical examinations and genotypic by using 16SrRNA gene. The relationship between S. aureus biofilm producer isolates and icaABCD operon genes was studied by using PCR. Twenty four (24) isolates of pathogenic S. aureus bacteria, which showed biofilm production were selected for genetic detection of the presence of biofilm related genes, including intracellular adhesion (ica) genes, which are within the biological operator icaABCD using polymerase chain reaction (PCR). The detection results showed that 20 out of 24 isolates under study 83%, were carriers of the icaA and icaB genes, and the percentage of the icaC gene in the isolates was 17 (70%), as well as the rate of icaD gene in the isolates under study was 19 (79%). The genetic patterns of the isolates that possess biofilm encoding genes showed that 17 (70.8%) isolates contained the type icaABCD, 2 (8.3%) isolates showed the type icaABD, one isolate by 4.2% showed the type icaA, and one isolate showed the type icaA. type icaB by 4.2%, and three isolates did not show any pattern because they did not have icaABCD operon.