Comparative Evaluation for the Anti-Inflammatory & Anti-Oxidant Properties of Hydromethanolic Extract of Leaves, Flowers and Stems of Combretum Indicum Linn in Albino Wistars Rats

Abstract

Combretum indicum plant indicated as herbal medication for many of diseases and disorders due to presence of various pharmacologically active phytoconstituents such as pelargonidin 3-glucoside, quisqualic acid, pelargonidin,Trigonelline and rutin. In this study, ratio of 50:50 Hydro-methanolic Extract of leaves, flowers and stems were taken and comparative evaluation was done by performing 21 days of CFA induced chronic anti-inflammatory model in female albino wistar rats. Preliminary phytochemical examination confirmed about the presence of phytocomponents such as carbohydrates, Tannins, flavonoids, glycosides, oils & fats, amino acids & protein which are already mentioned. 400 mg. kg.body weight selected extracts of these three parts of C.indicum were given to animals after examined acute oral toxicity. Paw volume, paw diameter, Body weight, arthritis score were examined on 0, 7^th , 14^th and on 21^st days of model. Examination of blood components (RBCs, WBCs, PLTs, Hb, RF factor and CRP and Biochemical markers (SGPT, SGOT and ALP) were done with the blood, for enzymatic activity (SOD, CAT, GPx, GSH) antioxidant study done with blood.  Histopathological evaluation & radiograpgy of animals paws were done in the end of the study. Improvement like condition in these parameters, confirmed the explanation of anti-inflammatory and antioxidant activity of this extract  with compare to other, leaves extract was found more effective.