Genetic Association of TCF7L2 Polymorphisms (rs7901695 and rs7903146) with Type 2 Diabetes Mellitus in East Godavari, Andhra Pradesh, India

Abstract

Objectives: This paper aims to compare T2DM and genotype connection in the TCF7L2 gene particularly the two SNP’s that are, rs7901695 and rs7903146. The kind of research design used to undertake this study is a comparative analysis between 150 T2DM patients and 150 healthy individuals from the area of East Godavari, Andhra Pradesh, India.

Materials and Methods: A case-control study was done in 150 T2DM patients and 150 ethnically matched normal controls. Integral blood samples and genomic DNA were obtained, respectively, and TCF7L2 polymorphisms were detected by using real-time PCR. Statistical information was processed using the help of SPSS and comparisons were made between the demographic, lifestyle, and genotypic and allelic status of the two groups.

Results: T2DM patients were older, and more obese with a higher BMI value and parity than the control group of women. They also reported a higher rate of hysterectomies, a later age of menarche, and more. There were no other differences between the two groups about other demographic measures and lifestyles. Population genetic stratification and analysis of the allelic distribution of SNPs rs7903146 (OR=0.0075) and rs7901695 (OR= 0.0091) made it possible to establish the relationship between the T allele of the rs7903146 marker and the C allele of the marker rs7901695 with T2DM.

 Conclusion; T2DM relates to different factors including age, weight, parity and abortions, age at menarche, menopausal status, and presence of other diseases. SNP analysis revealed that rs7903146, and rs7901695 of the gene TCF7L2 are strongly linked to T2DM.