Hepatoprotective Effect of Methanolic Extract of Origanum Majorana on Paracetamol-Induced Liver Toxicity in Rats

Abstract

Origanum majorana is a medicinal plant with traditional claims but ignored investigation regarding its hepatoprotective effects. The current study is aimed to investigate the hepatoprotective potential of Origanum majorana methanol, water extract and isolated compound (hesperetin) on paracetamol-induced liver injury. The phytochemical screening was done which showed the presence of potential constituents including flavonoids and phenols. For investigating the hepatoprotective effect of Origanum majorana extract, rats were given different treatments for seven consecutive days. The normal control was administered with normal saline, group 2 received paracetamol and group 3 (Standard) was given silymarin as reference drug. In group 4 and 5 (Treated), methanolic extract (100 and 200mg/kg); group 6 and 7 (Treated) aqueous extract (100 and 200mg/kg); group 8 (hesperetin) were administered. Different serum biomarkers and histopathological studies were performed to assess the recovery caused by paracetamol in comparison to diseased group. The treatment of Origanum majorana methanolic extract and (hesperetin) significantly improve the serum biomarkers and restored the hepatic injury towards normal, indicating the hepatoprotective potential. Thus, we can conclude that Origanum majorana and isolated compound (hesperetin) have significantly reversed the damage caused by paracetamol in hepatotoxic rat model