Development and Validation of RP-HPLC And HPTLC Methods for the Quantification of Curcumin in Tablet Formulation.

Abstract

Introduction:Curcumin tablets are herbal formulations based on Ayurveda that are used to treat arthritis and boost immunity. In this study, a novel and precise method for measuring curcumin in Curcumin tablet formulations was developed and validated.

Objective:The objective of the study was to create specific and accurate methods using RP-HPLC and HPTLC techniques to measure curcumin content in Curcumin tablet formulations according to ICH Q2(R1) guidelines.

Methods:Development of the methods engaged use of linomat IV sample spotter precoated TLC plate for HPTLC analysis. Toluene, ethanol, and formic acid were employed as mobile phase compositions with 8.6:0.7:0.7% v/v ratio for HPTLC while HPLC analysis used an acetonitrile, methanol, water (40:30:30) mobile phase at a flow rate of 1mL/min with maximum detection wavelength at 425 nm using C18 ODS column (250×4.6 mm, 5 μ). The linearity ranges for HPTLC and HPLC were found out as 600-1400 ng/spot and 10-50 µg/ml respectively. Rf values along with retention times were recorded.

Results:Accordingly, the Rf value was determined as 0.321±0.03 by hptlc while retition time was observed as9.287 by hplc method.The methods could accurately analyze the amount of curcumin in Curcumin tablet formulations within their respective linear ranges defined.

Conclusion:RP-HPLC and HPTLC methods are developed for quantification of curcumin in tablet formulation which are precise and can be used for routine analysis.