Effective Diabetic Foot Ulcer Healing Potential of Isolated Asperesocoumarin Compound from Fungal Endophyte

Abstract

Diabetes was a metabolic disorder characterized by prolonged hyperglycemia that is affecting approximately 500 million populations leading to over 5millions deaths. DM results in complications like retinopathy, nephropathy, neuropathy and especially foot ulcer that are leading to amputation and disability to majority of population. Natural products are ideal to treat the foot ulcers, and fungi serve as critical sources of the molecules that treat diabetic foot ulcers effectively. Thus this research is designed to isolate coumarin molecule from fungal endopytes and to investigate the anti-diabetic activity of Asperesocoumarin-B (Ac-B) and related healing of foot ulcers in STZ+NIC induced diabetic model. The isolated molecule showed better anti-diabetic activity in terms of lowering the blood glucose levels, oral glucose tolerance, controlling the body weight, as well as normalizing liver and kidney function parameters. The isolated molecule also showed significant normalization of antioxidant enzymes and lowering of oxidative LPO. The isolated molecule (AC-B) showed increase in wound healing of the diabetic rats in terms of reduction of the wound size over 12 days and also increase in total protein content. Histopathological studies on liver tissue and wound tissue showed significant healing and preserved tissue architecture and integrity. Healing effects were attributed in part to reduced free radical damage, the promotion of antioxidant status, the rapid deposition of collagen, and the anti-diabetic activity.