Design, Synthesis, Characterization and Antimicrobial Activity of Schiff Base Containing N, N’-di substituted Benzimidazole-2-Thione Derivatives

Abstract

T Medicinal chemistry involves the discovery, development, identification and interpretation of the mode of action of biologically active compounds at the molecular level. The Aim of this research work is to design, synthesis, characterization and antimicrobial activity of Schiff base containing N, N’-di substituted Benzimidazole-2thione derivatives. In the present work, different analogs of Schiff base containing N, N’di substituted Benzimidazole-2-thione derivatives (3a-3f) were designed and subjected for docking study using free available software Argus lab 4.0. Among the designed compound, substituted benzaldehyde containing the functional group like (OH, NO₂, OCH₃ and Cl) and un substituted compound was found to showed good binding energy as compared to another and also the compound substituted with-Cl group shown better binding energy. Furthermore, among the designed compounds which possess good binding energy was selected for synthesis and the new series of different benzaldehyde containing N, N’-di substituted Benzimidazole-2-thione derivatives were prepared by treating intermediate-2 with substituted benzaldehyde by suitable synthetic method. The synthesized new compounds were purified by recrystallization method using suitable solvents and the structure of newly synthesized compound was confirmed by physicochemical and spectral analysis like IR, ¹HNMR, ¹³CNMR and molecular weight of compound was confirmed by GC-MS spectroscopy. On the basis of interpretation of spectral data obtained from spectral analysis, it was concluded that the synthesized compounds were desired compounds i.e. 1H-benzo[d]imidazole-2(3H)-thione-1,3bis(methyl urea) benzylidine) (3a-3f) as expected. Subsequently in the anti-microbial screening, almost all the test compounds exhibited significant antimicrobial activity against both gram positive and gram negative bacteria and synthesized compounds was considered as promising lead candidate for development of new antimicrobial agents.