Assessment of Cytotoxic Effect of Para-probiotic Yogurts on Cancer Cell Lines

Abstract

Background and Aim: Dairy products enhanced with para-probiotics and postbiotics offer a more convenient and streamlined option for industrial transportation and commercialization compared to probiotic products. Various sections of Lactobacillus, including whole cells, thermally inactivated cells, cell wall, peptidoglycan, and cytoplasmic sections, have demonstrated antiproliferative effects against human cancer cell lines. This study investigated the impact of para-probiotic yogurt on cancer cells. If proven effective, it has the potential to be utilized in the treatment of this form of cancer as well as other types of cancer.

Material and Methods: Following the culturing and amplification of the cancer cell line, an investigation was conducted to assess the impact of supernatant toxicity. The cells were then subjected to varying doses of para-probiotic supernatant, with the MTT assay being performed 72 hours later.

Results: The experiment involved testing the effects of various dilutions of para-probiotic yogurt supernatant on different types of cancer cells (MDA-MB-231, SK-BR-3, and SW-480). The results showed that YBpB had a significant inhibitory effect on cell viability in all three categories of cancer cells. Notably, it had the strongest impact on SK-BR-3 cells (p≤0.05). The concentration of the extract had a significant impact on the cytotoxicity effects, with higher concentrations resulting in increased growth inhibition. The highest percentage of growth inhibition was observed at concentrations of 1/4, 1/3, and 1/2, with statistical significance at p = 0.05 and p = 0.01, respectively.

Conclusion: It is recommended to consider the use of para-probiotic yogurt supernatant as an effective substance in the treatment of cancer. Future research could investigate the potential use of its substances in cancer treatment.