Bioactive Components in Moringa Oleifera: Phytochemical Screening, Gc-Ms Profile, in-Vivo and in-Silico Components Against Anti-Depressant Activity

Abstract

Introduction: Moringa oleifera (moringaceae) has been known since ancient times for its rich medicinal properties. Using both in vivo and in silico research, the effects of the ethanol extract of Moringa oleifera leaves (EOL) on depression were assessed.

Objectives: The goal of the current research was to evaluate Moringa oleifera's (MO) antidepressant efficacy using a mouse model

Methods: Female albino Wistar rats were divided into five groups, taken for the study and dosed once with 2000 mg/ kg of the extract of EOL are administrated orally gavage. The antidepressant activity was evaluated using forced swim test (FST) and tail suspension test (TST).

Results: The in-silico studies included molecular docking against GPCR and PI3K protein. The results of in vivo studies showed that both AC (0.10, 0.20 and 0.40 g/kg, p.o.) and imipramine (15mg/kg, i.p.) significantly decreased the duration of immobility time in a dose dependent manner in FST model and the outcomes demonstrated that both MO (100,200,400 mg/kg, p.o.), imipramine (15 mg/kg, i.p.) significantly shortened the duration of immobility time in a dose-dependent way in TST model. The in-silico studies results are showed best docking score against the targeted compound respectively.

Conclusions: Moringa olifera showed antidepressant activity since it reduced the immobility in both FST and TST. Our results also confirm the involvement of serotonergic, noradrenergic and dopaminergic pathways in depression. Pretreatment with Moringa olifera, also significantly increased the SOD levels and Catalase with simultaneous decrease in LPO levels in rat brain, suggesting its strong antioxidant activity. Since oxidative stress is reported, plays important role in depression, the antioxidant activity of Moringa olifera may contribute to antidepressant activity.