Cytotoxicity and Electrochemical Study the Effect of ALF3 Incorporation to Acrylic Resin Materials

Abstract

Aluminum fluoride (AlF3) was one of the compound that treated with different denture composition, also used incorporation with acrylic resin  to improve and support the physical and chemical properties.

Aim: to find the electrochemical analysis by cyclic voltammetric (CV) technique, the physical properties of these material and the effected in artificial saliva (AS) by oxidation – reduction peak current in oral cavity. Also to Evaluate the cytotoxicity effect of ALF3 incorporation to acrylic resin materials

 Material and method: for electrochemical analysis used glassy carbon electrode (GCE) as working electrode, Ag/AgCL as reference electrode, and platinum wire as axillary electrode in cyclic voltammetric cell. Different chemical properties such as concentration, pH, scan rate, and reproducibility study were used in potentio-state. 

Ten samples incorporated with different concentration of different fluoride salts as follow: 2%AlF3 and 5%AlF3 ,five samples for each concentration, all these specimen cultured for 24h,48h and 72h.and test for mtt test).

Results: Oxidation peak current was appeared in the voltammogram of AlF3 in artificial saliva at 125 mV which was studied the electrochemical behavior at different ways. The oxidation peak was gradually enhanced by increasing the concentrations and scan rates that give a calibration curve with high sensitivity, but in different pH the oxidation peak of AlF3 was disappeared in alkaline pH and enhanced in acidic medium, so the AlF3 ions act as oxidative reagent in artificial saliva, and antioxidant in alkaline pH, so the low concentration of 2%AlF3 in the acrylic was more favor than high concentration also addition of 2% and 5% AlF3 to heat cured acrylic resin materials showed no effect on the cytotoxicity test.

Conclusion: In the high concentration of 5%AlF3 has been affected in oxidation behavior more than low concentration of 2%AlF3. In the acidic pH of the artificial saliva has high intensity of oxidative property which act damage to the cell of mouth while in the alkaline pH of artificial saliva act as antioxidant behavior that can be used in safety .The addition of 2% and 5% of AlF3 into heat cured acrylic resin materials showed  no toxic effect.