Microbialactivity, Phytochemical Constituents and Total Phenolics Content of Physalis Angulata

Abstract

Introduction: One of the primary reasons of this issue is the widespread occurrence of acquired bacterial resistance to antibiotics, which poses a major threat to public health worldwide today in the form of pandemics and epidemics of antibiotic resistance.

Objective: This current research project involves conducting phytochemical, pharmacognostical, and antimicrobial studies on Physalis angulata.

Methods: The plant material in powdered form was extracted using different solvents including ethanol, ethyl acetate, chloroform, and water. The solvent extracts were comprehensively characterized through a series of evaluation tests, including phytochemical evaluation through chemical tests, proximate analysis, for bioactive compounds present in the extract. Furthermore, the antimicrobial potential of the extracts was evaluated using disc diffusion method. The antimicrobial activity was further assessed by determining the minimum inhibitory concentration.

Results: Result revealed that presence of alkaloids, glycosides, flavonoids, terpenoids, and phytosterols in ethanol solvent and other solvents contain limited bioactive compounds. Proximate analysis data showed presence of moisture of 9.42 %, total ash content 8.47 %, ethanol soluble extractive value 7.84%, water soluble extractive value 4.56%. Further, total phenolic content was estimated and revealed presence of 256.23 mg/g plant extract (GAE). Finally, antimicrobial activity of the extract was performed against Gram positive (S. aureus and B. subtilis), Gram negative (E. coli, and Pseudomonas aeruginosa) bacteria and two fungal organisms Aspergillus niger, Candida auris after performed MIC and MBC tests.

Conclusion: Result revealed dose dependent antimicrobial activity and finally result correlated with the content of total phenolics which showed positive correlation.