Pharmacological Evaluation of Antioxidant Potential of Hydro Alcoholic Extract of Triticum aestivum (Wheat Grass)

Abstract

The present study was based on the pharmacological evaluation of antioxidant potential of 
hydro-alcoholic leaves extract of Triticum aestivum (Wheat grass). T. aestivum was grown 
under indoor conditions in Bareilly. The plant was authenticated by Dr. Alok Srivastava 
(Associate Professor) Plant Science. The authentication letter reference no. RU/PS/19/03 as 
issued from department of plant science MJP Rohilkhand University, Bareilly. Dried 
powdered leaves (50gm) and root (50gm) were extracted separately with Soxhlet apparatus 
using 500 ml of hydro-alcoholic solution (1:1) ethanol: water for 24 hrs. All the animals 
were divided into 4 groups (n=6) i.e., group I (control) rats treated with vehicle only (0.5% 
carboxymethylcellulose sodium, p. o.). Group II, III, and IV rats administered leaves extract 
of Triticum aestivum (100, 200, and 400mg/kg, p. o.), respectively, for 7 days. Blood 
samples of the experimental animals was collected by retro orbital artery bleeding after 16 
hr of the last dose. Blood samples were centrifuged for 10 minutes at 2000 rpm to separate 
the serum. Triticum aestivum leaf extract evaluated for GSH, CAT, LPO, and SOD. In 
results, upon calculation, the % yield for Triticum aestivum leaves and root extract was 
obtained as 54% and 18%, respectively. (Triticum aestivum leaf extract) the % antioxidant 
activity was observed as 24.56*%, 25.21*%, 39.10*% and 64.85*% in the concentration of 
50µI, 100µI, 150 µI and 200 µI, respectively. SOD level was observed as 3.84±1.41 U/mg 
protein, 4.07±0.13 U/mg protein and 4.10±0.61 U/mg protein in group 2, 3 and 4, 
respectively which exhibited the significant decrease in SOD level. In conclusion, a potent 
antioxidant and modulation effects in CAT, GSH, and TBARS by the hydroalcoholic leaves 
extract of T. aestivum. In future aspect, it suggests to determine the effectiveness and safety 
of T. aestivum hydroalcoholic leaves extract as a possible natural antioxidant source.