Formulation and characterization of azacitidine nanoemulsion for the treatment of tumor suppressor genes activity in myeloid leukaemia cell line
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Abstract
The goal of the current study is to define and developed an Azacitidine nanoemulsion. Azacitidine is a derivative of pyrimidine nucleoside that prevents DNA methyltransferase from working properly, hence affecting DNA methylation. Incorporated largely into RNA, it additionally functions as an antimetabolite of cytidine. The anti-cancer drug azacytidine has been utilized and absorption rate is very minimal. Thus, nano-emulsions have been formulated in order to address these problems. Nano-emulsion made up of distilled water, plysorbate 80, Azacitidine, various oils (including oleic acid), and co-surfactants Transcutol P. By using the high-speed homogenization method, various water-in-oil nano-emulsion are formulated. Thermodynamic stability testing, viscosity test, pH test, FTIR test, in-vitro drug release test, and stability testing were used to evaluate the nano-emulsion formulations.Methotrexate, a standard medication, and nanoemulsion were found to have the greatest % inhibitions, 89.09% and 85.37%, correspondingly.Given that it has been shown that AZT nanoemulsion may also function to block histone deacetylases, we propose that it may one day be employed as a therapeutic option in myeloid leukaemia cell lines.