Formulation and characterization of azacitidine nanoemulsion for the treatment of tumor suppressor genes activity in myeloid leukaemia cell line

Abstract

The goal of the current study is to define and developed an Azacitidine nanoemulsion. Azacitidine is a derivative of pyrimidine nucleoside that prevents DNA methyltransferase from working properly, hence affecting DNA methylation. Incorporated largely into RNA, it additionally functions as an antimetabolite of cytidine. The anti-cancer drug azacytidine has been utilized and absorption rate is very minimal. Thus, nano-emulsions have been formulated in order to address these problems. Nano-emulsion made up of distilled water, plysorbate 80, Azacitidine, various oils (including oleic acid), and co-surfactants Transcutol P. By using the high-speed homogenization method, various water-in-oil nano-emulsion are formulated. Thermodynamic stability testing, viscosity test, pH test, FTIR test, in-vitro drug release test, and stability testing were used to evaluate the nano-emulsion formulations.Methotrexate, a standard medication, and nanoemulsion were found to have the greatest % inhibitions, 89.09% and 85.37%, correspondingly.Given that it has been shown that AZT nanoemulsion may also function to block histone deacetylases, we propose that it may one day be employed as a therapeutic option in myeloid leukaemia cell lines.