The Effect of Radiation, Chlorine and Sulfate Salts on the Activity of Carbonic Anhydrase Enzyme in the Ontogenesis of the Cotton Plant

Abstract

Article deals γ-radiation doses of 5, 10, 50, 100, 200, 300 Gy, chloride (NaCl, FeCl3) and sulfate (Na2SO4, ZnSO4) concentrations of 5, 10, 50, 100, 200, 300 mM of cotton Gossypium hirsutum L. The dynamics of changes in the activity of carbonic anhydrase (CA, carbonate hydrolyase, EC 4.2.1.1) enzyme were studied in the ontogeny of the Ganja-182 cultivar of the genus Ganja-182 in type of true leaf emergence (LP), budding (BP), flowering (FP) and opening of seed boll phase (OBP). It was determined that chloride and sulfate salts have different effects on CA activity. Thus, CA activity increases at 200 Gy dose of γ-radiation, 100 mM of NaCl, and 200 mM of ZnSO4. It seems that the increase in CA activity in the medium containing ZnSO4 is related to the increase in the demand for CO2 under stress. The obtained results show that radiation and sulfate salts have a more regulatory effect on salt adaptation than chloride salts in cotton plant.