A Study on the Bioaccumulation Potential of Two Bacteria Isolated from Chettipunyam Lake and Thirukachur Lake water samples of Chennai city

Abstract

Aim: The present study was aimed to isolate and investigate the bioaccumulation potential of two bacteria isolated form Chettipunyam Lake and Thirukachur Lake, in Chennai City.

Methods: Isolates were identified using standard 16srRNA sequencing and DNA amplification methods. Sample water was collected and physio-chemical characteristics was tested as per APHA standards. Evaluation of bacterial strains for the removal of heavy metals (Zinc, Iron and Copper) from lake water samples was determined using standard methods.

Findings: Two significant metal chelating bacterial cultures isolated from Chettipunyam Lake and Thirukachur Lake was identified as Priestia megaterium CL3 and Bacillus cereus CL4 16S respectively. Physico-chemical parameters tested as per APHA standards showed that, all three metals, Zn, Fe and Pb was found approximately 1050-1450 folds higher than that of permissible level in both lake water samples. The obtained percentage loss by standard strains when compared to the percentage loss efficiency by the consortium isolates showed almost similar level in percentage loss of maximum up to 89.9% after 60 hours; and up to 92.6% after 120hours.

Conclusion: The findings revealed the potential ability of bacterial isolates for better metal accumulation efficiency from any type of water or waste water samples. As a future study, the treated or metal accumulated water shall be used for basic agricultural activity in small fields where water is not needed in more quantity.