Survey of Mycotoxins in Wheat from Iran by HPLC Using Immunoaffinity Column Cleanup

Abstract

Mycotoxins are small, toxic chemical products produced as secondary metabolites bya few fungal spices that readily colonise crops and contaminate them with toxins in the field or afterharvest. In this study a liquid chromatographic method was developed for the simultaneousdetermination of aflatoxins (AFs) (B , B , G , and G ), ochratoxin A (OTA) and zearalenone(ZEA)toxins in wheat. For this purpose, a total of wheat samples were analyzed. Mycotoxins wereextracted and purified from the samples using immunoaffinity (IAC) columns. and ofexamined wheat samples contained AFB and OTA. The method was based on the extraction ofAFs, OTA and ZEA finely ground wheat sample with methanol and acetonitrile solution,respectively. and of examined wheat samples contained AFB and OTA. LOD was, , , , and for AFB , AFB , AFG , AFG , OTA and ZEA,respectively.Considering these results, these special products should not be a health concern forthese collectives; however, special attention needs on foods distribution in retail shops.