Antimicrobial Profile of Synthesized Β-Cyclodextrin-Isatin (Schiff’s Bases) Inclusion Complexes

Abstract

Isatin derivatives are vulnerable known motifs by virtue of diversified bioactivities and synthetic applicability. Herein assorted Isatin Schiff’s bases as 1-(2-oxoindolin-3-ylidene)thiosemicarbazide (G1) and  3-((4-chlorobenzylidene)hydrazono)indolin-2-one were assembled and characterized for innate potent antimicrobial profile. Moreover, allied inclusion complexes with β-Cyclodextrin were prepared so as to attain augmented pattern of pharmacological profile. Synthesized Isatin derivatives exhibited UV λmax 373nm and 410 nm for G1 and G2 respectively,  FT-IR and 1H NMR data that validated Schiff’s base formation. β-Cyclodextrin-Isatin (Schiff’s bases) inclusion complexes were prepared by co-precipitation method and verified by preliminary physico-chemical examinations such as colour changes, alteration in melting point etc. and further confirmed by spectroscopic techniques like FT-IR and 1H NMR.  Antimicrobial profile demonstrated significant bioactivity against bacterial strains, with minimum inhibitory concentrations (MICs) range 10-50 μg/mL. Certain inclusion complexes showed potent bioactivity against Candida albicans, with MICs as low as 20 μg/mL. The fruitful synthesised and characterized Isatin derivatives and allied inclusion complexes with β-CD showed enhanced antimicrobial profile against assorted pathogens like Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Candida albicans. These research findings highlighted the worth of Isatin derivatives and allied inclusion complexes as prominent motifs in order to develop significant pharmaceutical formulations, particularly in combating microbial infections and diseases.