Anti-oxidant Activity and Biochemical Evaluation of Rice Bran

Abstract

Introduction: Rice is very commonly used food product all over the word, Asia, China, India and other country. It is used as food from ancient time. Rice bran obtained from milling of rice traditionally used as a medicinal product for treatment of disease and journal purpose. Rice bran obtained from various rice verities are used for treatment of various disease and cosmetic product. Several study stated that the chemical present in rice bran is used for treatment of various disease like diabetes, cancer, heart disease and beautifying agent etc. chemical present in rice bran like tocol and oryzanol, Phytosterol, Tocotrienol, fiber and Oryzanol help in lowering cholesterol, anti-cancerous and cardioprotective effect. Extract of bran exhibit antioxidant property due to presence of phytochemicals phenols, pigments, oryzanol and tocols and anti-carcinogenic property due to presence of hemicelluloses and Tocotrienol.

Objectives: The objective of this study was to investigate the phytochemical present on rice bran by various chemical test of bran extract and in-vitro study of antioxidant activity. Anti oxidant activity study by using DPPH (2’2-Diphenyl-1-picrylhydrazyl) assay method and Nitric oxide scavenging activity method

Methods: Study involved step by step process and various method to determined its phytochemicals and antioxidant property. extract was obtained by using soxhlet extraction process. These  extract was used for phytochemical screening, identification of the various organic compound, inorganic compound and other active constituents like alkaloid, Flavonoide, Glycosides ,Steroid, Protein  and Carbohydrate. Free radicle scavenging activity determined by the help of DPPH (2’2-Diphenyl-1-picrylhydrazyl) assay method and Nitric oxide scavenging activity method.

Results: The study of various phytochemical was done by soxhlet extraction process. the obtained extract of rice bran from petroleum ether, ethanol solvent used for the study. Extractive value of  prtrolium ether and ethanol was found to be 4.8% and 9.7 % respectively. The phytochemical screening show the presence of alkaloid, Flavonoide, Glycosides ,Steroid, Protein  and Carbohydrate and inorganic constituents like calcium, magnesium, potassium iron and absence of phosphate, chloride, sulphate. DPPH and nitric oxide scavenging activity based on the concentration of the rice bran extract. Ethanolic fraction of rice bran useful for reducing free radicle related cell damage..

Conclusions: Rice bran contains significant medicinal value due to presence of various bioactive compounds like glycoside flavonoide alkaloid etc.. Hydro-alcoholic extract of Rice bran use to reveals that the extract contain various bioactive compound and exhibit significant antioxidant activity. Further in-vivo study of extract help to better understanding of the traditional clam of Oryza sativa as a medicinal plant.