Isolation and Extraction of Artificial Sweetener (Stevia) Using Stevioside Conventionally

Abstract

Introduction: The herb The Bertoni Stevia rebaudiana family (asteraceae or compositae) is used to extract stevioside from its leaves. It serves as a natural, minimal-calorie sweetener because of its pleasant flavour. These glycosides have a broad range of medicinal applications and possess 300 times the sweetness of sugar. Furthermore, Dietary carbohydrates are abundant in stevia plants. (60.92% d.w.), amino acid (12.42% d.w.), raw cellulose (14.53% d.w.), elements (a K-22.16; Ca-16.6; The sodium-15.92; Manganese-3.25 mg/100gm d. wright.; Copper-0.73; Manganese-2.89; the iron-5.89; Zn-1.25 mg/100gm d.w.), plus cholesterol. In many places, it is used in place of sucrose in food and beverages.

Objectives: Identify natural phytoconstituents that can be be use for sweetening agent in Bertoni Stevia rebaudiana.

Methods: Isolation of Bertoni Stevia rebaudiana done by Soxhlet apparatus and then the extract undergoes TLC Chromatography study for isolation

Results: Using two distinct solvents with Rf values of 0.50 and 0.67 on TLC, stevioside was effectively separated. It was then further purified and recrystallized.

Conclusions: - The low-calorie steviol glycosides called steviosides are found in its Rebaudiana Bertoni the plant's leaflets.They are frequently used by diabetic people as a natural sweetener. The stevioside band is visible in the TLC data, and this is verified by contrasting the samples' Rf value (0.50) with the standard's.