GCMS analysis and Hepatoprotective effect of Impatiens henslowiana on Galactosamine -Induced Hepatotoxicity in HepG2 Cells

Abstract

Introduction: The liver is a vital organ in the human body, performs numerous essential functions, rendering it vulnerable to various liver diseases. Damage to the liver typically involves cell death, increased oxidative stress, and decreased levels of glutathione, a crucial antioxidant. Despite advancements in medical science, liver diseases continue to pose a significant global health challenge. Conventional drugs used to treat liver diseases often fall short and may have adverse effects. Therefore, there is a growing interest in exploring alternative treatments, particularly those derived from traditional medicinal plants.

Objectives: This study aimed to validate the hepatoprotective activity of extracts from Impatiens henslowiana against Galactosamine-induced hepatotoxicity in HepG2 cells.

Methods: The cells were exposed to different extracts at concentrations ranging from 1.56 μg/ml to 25 μg/ml in combination with Galactosamine (0.1%) for 24 hours. The hepatoprotective effects were assessed through various assays, including cell proliferation and the oxidative stress mechanism.

Results: The anti-proliferative effect was enhanced in the presence of all plant extracts at all tested concentrations. The ethanol extract-treated HepG2 cells exhibited a 64.45% reduction in the Galactosamine-induced group. Galactosamine led to a 198.25% increase in MDA level, which was attenuated by the ethanol extract at 169.34% at 6.25 μg/ml. GSH content in cells decreased by 52.25% with Galactosamine, but the plant extract increased GSH levels by 64.38%. Additionally, Galactosamine induced a 34.26% decrease in SOD levels, but this necrotic effect was diminished by the treatment group to 51.62%.

Conclusions: In conclusion, extracts from Impatiens henslowiana demonstrated a preventive effect on HepG2 cell injury induced by Galactosamine treatment for 24 hours, and the hepatoprotective activity of the ethanol extract was comparable to that of the standard drug silymarin.