Assessment of Salivary Stress Marker (Peroxidase) Levels in Completely Edentulous Patients: crosssectional study

Abstract

BACKGROUND :

Patients' diet quality and nutritional status decline as a result of their avoidance of hard meals, including both vegetables and non-vegetables, due to their reduced ability to chew due to tooth loss. Peroxidases are either directly or indirectly associated with several major human disorders, including cancer, periodontal disease, convulsive illnesses, Parkinson's disease, and coronary artery disease (CAD). These diseases can arise as a result of a variety of different chemicals that are involved in the metabolic pathways of peroxidase action, such as auto-antibodies, flavonoids, and thiocyanates. Recent research provides indirect support for the notion that dental implant therapy-based oral rehabilitation is beneficial for people with diabetes. In both diabetic and non-diabetic fully edentulous individuals, we assessed salivary glucose levels to see if the diabetic patient may have any detrimental effects on issues after surgery, implant failure, and peri-implantitis.

AIM: The present study aims to evaluate the peroxidase levels in completely edentulous patients.

MATERIALS AND METHOD: Add the sample and reagent into each well of the human lactoperoxidase ELISA kit. At 37 degrees, incubate for one hour. Rinse the plate five times. Combine solutions A and B for the substrate. Incubate at 37 degrees for ten minutes. Colour develops when a stop solution is added. Within ten minutes, determine the OD density value.

RESULTS AND DISCUSSION: According to the current investigation, there was a statistically significant difference in the groups' baseline means (p=0.00). Patients with diabetes had a mean value of 3.09, and for those with diabetes, it was 2.77, both of which are statistically significant.

CONCLUSION: A significant increase was observed in peroxidase levels in the completely edentulous patients but an elaborate study needs to be conducted with a larger sample size.