High Neutrophil - Signal Fluorescence Intensity (NE-SFL) Values as Predictor of Sepsis Occurrence in Adult Patients

Abstract

Background: Sepsis is a systemic response to infection. Neutrophils play a crucial role in fighting microbial infections, thereby reducing the presence of pathogenic organisms within the body. Neutrophil - Signal Fluorescence Intensity (NE-SFL) is one of the parameters that make up the cell population data (CPD). This parameter can be obtained using the Sysmex XN hematology instrument. NE-SFL helps measure neutrophil immaturity and activation. Specifically, high fluorescence intensity indicates increased DNA or RNA content, reflecting significant cytokine production.

Objective: To determine the significance of elevated NE-SFL as a predictor of sepsis in adult patients.

Methods: This study is an analytical observational research with a case-control design involving 77 individuals. The study was conducted from September 2022 to March 2023 at Prof. Dr. I. G. N. G. Ngoerah Denpasar Hospital.

Results: The study included 37 sepsis patients and 40 non-sepsis patients. Data analysis revealed that sepsis patients had higher NE-SFL values than non-sepsis patients, with a p-value of 0,000. Based on the ROC curve, the NE-SFL cutoff value as a predictor of sepsis was 46,4 (95% CI: 0,743-0,440, p < 0,001). The odds ratio (OR) of NE-SFL for sepsis was 24,286 (95% CI: 7,345-80,297, p < 0,001). Neutrophil - Signal Fluorescence Intensity was considered meaningful as a predictive model for sepsis in adult patients.

Conclusion: In this study, neutrophil - Signal Fluorescence Intensity can be used as a predictor of sepsis in adult patients