Rapid Decontamination of Gutta-Percha Points- An in Vitro Comparative Study between Different Auxiliary Chemical Substances

Abstract

Introduction: Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Vitae sapien pellentesque habitant morbi tristique senectus et netus. Dignissim cras tincidunt lobortis feugiat vivamus at augue eget arcu. At risus viverra adipiscing at in. Cras semper auctor neque vitae tempus quam. Sed cras ornare arcu dui. Turpis massa sed elementum tempus. Risus commodo viverra maecenas accumsan lacus vel facilisis volutpat est. Dictum non consectetur a erat nam at. Lorem mollis aliquam ut porttitor leo. Egestas sed sed risus pretium quam.

Objectives: To compare and evaluate the efficacy of 3% Sodium Hypochlorite, 2% chlorhexidine, 0.1% Octenidine Dihydrochloride and 10 h milled ZnO nanoparticle in Disinfection of Gutta Percha Cones against E. faecalis in vitro

Methods: A total of sixty gutta-percha cones taken from a freshly opened sealed packet of size 70 and 2% taper were used. They were divided into various groups according to the type of disinfectant. The GP cones were contaminated with microbial suspension of E. faecalis and then decontaminated by immersing in the different disinfectant solutions for 1 min. GPs were transferred to test tubes containing thioglycollate media and incubated at 37°C for 7 days. After 7 days, the media was transferred to a petridish containing brain heart infusion (BHI) agar and incubated for 48 h aerobically at 37°C and the colony forming units (CFU) were counted with digital colony counter.  Descriptive data was explored in terms of mean and standard deviation. The data was statistically analyzed by was Kruskal Wallis Test using SPSS version 25.0 with significance level kept at p<0.05.

Results: The least number of colonies was seen for 5.25% NaOCL while the maximum mean number of colonies was seen for positive control group.0.1% octenidine dihydrochloride also showed non-significant difference with 5.25% NaOCl.

Conclusions: It was observed that immersion of gutta-percha into 5.25% NaOCL for one minute was found the most effective method to eliminate the selected microorganisms followed by 0.1% octenidine dihydrochloride.