Comparative In-Vitro Evaluation of Herbal Extracts for Synergistic Effects of Anti-Inflammatory and Antioxidant Activity

Abstract

Introduction: In the current work, in-silico docking investigation for 60 benzopyran-3-carbonyl derivatives was conducted since benzopyran derivatives play a vital role in many different research disciplines. The anti-arthritic activity of polyherbal extract was evaluated by in-vitro anti-inflammatory and in-vitro antioxidant activity. The anti-inflammatory activity was investigated by protein denaturation method using Bovine Serum albumin. Hydroalcoholic extracts of plants were used for evaluation at different concentration using Diclofenac sodium as reference drug at 660nm by using UV spectrophotometer. The in-vitro anti-oxidant activity of different hydroalcoholic extracts were measured by DPPH method using ascorbic acid as standard drug at 517nm using UV spectrophotometer method.The results revealed that all the herbal extracts possessed significant anti-inflammatory and anti-oxidant activities. But the maximum inhibition was obtained with the extracts when used in combination.  The polyherbal extract showed protein denaturation at 300 µg/ml concentration is 75.29412 % as compared to the standard drug diclofenac sodium, which showed 91.2156% inhibition. The polyherbal extract showed in-vitro anti-oxidant activity with inhibition of 60.37037 % and that of reference drug ascorbic acid 99.26852%.