Pedalium Murex Ethanolic Seed Extract Loaded Phytoniosomes Mitigates Monosodium Urate – Induced Gouty Arthritis in Rats

Abstract

The current investigation was carried out with the aim to investigate the anti gout arthritic activities of the ethanolic seed extract Pedalium murex (ESEPM)of and the ethanolic seed extractof Pedaliummurex loaded niosomes nESEPM in monosodium urate crystals induced gouty arthritic rats. The animals were pretreated with ESEPM and nESEPM (200 mg/kg b.w) for 10 days and gouty arthritis was induced on the 10thday.MSU crystals were infused into the synovial region on both sides of the ankle joint at a concentration of 80 mg/ml. The degree of protection by the ESEPM and nESEPM against the development of arthritis was evaluated by ankle swelling. The hematological and biochemical parameters were measured. The histopathological evaluation of the synovial membrane was also performed. In the current study the ESEPM and nESEPM drug treated rats exhibited low degree of ankle swelling compared to the MSU crystal induced arthritic rats. There was a significant reduction in the WBC cells and ESR levels as well as enzymes such as ALT, AST and xanthine oxidase was observed in MSU crystal induced and the ESEPM and nESEPM drug treated rat groups comparable to the MSU crystal induced rat groups. Histopathological  investigations are also in support of the protective nature of the ESEPM and nESEPM extract which shows the formation of less lesions in the synovial membrane in the drug treated group when comparable to MSU crystal induced groups.