Prevalence of Treponema Species and Periapical Pathology in Endodontic Retreatment Resistant Periapical Lesions

Abstract

Background: This study was conducted to assess the Prevalence of treponema species and periapical pathology in endodontic retreatment resistant periapical lesions

Material and methods: Clinical and radiographic examinations were used to select patients who need endodontic retreatment. Patients with systemic disease or those who had received antibiotic treatment during the previous three months were excluded from this research. Samples had been taken from 40 teeth with periapical lesions as well as endodontic failure, and clinical characteristics were documented. After separating the teeth from the oral cavity using a rubber dam, 30% hydrogen peroxide and 2.5% NaOCl were used to disinfect the teeth's exterior surfaces as well as the surrounding area. In order to prevent interference with bacteriologic sampling, the solutions were inactivated using 5% sodium thiosulfate. By obtaining a swab sample from the crown's exterior and streaking it on blood agar plates that were incubated both aerobically and anaerobically, the sterility of the crown's external surfaces was verified. There was a two-step access preparation process. Using species-specific 16S rRNA primers for T. denticola, T. amylovorum, and T. maltophilum, a second nested amplification was used to identify Treponema species. SPSS for Windows was used to statistically analyze the clinical feature data that were gathered from each patient and entered into a spreadsheet (SPSS, Chicago,IL, USA). To ascertain if there were statistically significant relationships between particular species and endodontic signs and symptoms as well as between lesion size and quantity of bacteria, including both positive and negative associations between the species, Pearson's chi-square or Fisher's exact tests were selected.

Results: A single negative sample was eliminated, whereas all other samples tested positive for bacterial DNA using the ubiquitous primer. Contrarily, the negative-control sample showed no signs of bacterial DNA presence, yielding no encouraging results. Out of 30 teeth, 16 showed the presence of Treponema denticola and 11 showed the occurrence of Treponema maltophilum. Treponema amyloyorum wasn’t evident. Treponema species was overall seen in 27/30 cases (90%).

Conclusion: In this study, from the above results it can be concluded that treponema species are present in endodontic retreatment resistant periapical lesions. In this study, these species were evident in 90% of the cases.