In Vitro Analysis of Guava (Psidium Guajava Linn) and Papaya (Carita Papaya L.) Leaves Extract Combination Against Escherichia Coli Growth

Abstract

Introduction: Incident diarrhea is still high in Indonesia; one reason for diarrhea is the existence of infection by Escherichia coli (E. coli). Still, many deep Indonesian societies resolve diarrhea by using herbal medicines such as guava leaves and papaya leaves.

Objectives: The objective of this study is to analyze in vitro analysis of guava and papaya leaf extract as antibacterial.

Methods: Actual experimental post-test only control group design, housed in the Laboratory of biomedical, Faculty of Medicine, Universitas Swadaya Gunung Jati Cirebon. Study this using a petri dish for growth bacteria E. coli, then give treatment study with combination leaf extract with concentrations of 100%, 75%, 50%, 25%, and 12.5%.

Results: The results showed that a combination leaf extract of 75% and 100% effectively inhibited growth comparable to E. coli bacteria with the effectiveness of ciprofloxacin. A 50% concentration can inhibit the growth of E. coli will but is far under the efficacy of ciprofloxacin.

Conclusions: Therefore, combination leaf extract with a minimum concentration of 75% effectively inhibits the growth of E. coli.