Extraction and Identification of Α-Chloralose in Biological Fluids (Gastrointestinal Fluids and Blood) By Solid-Phase Microextraction and Gas Chromatographies-Tandem Mass Spectrometry

Abstract

Introduction: Alpha-chloralose is a highly toxic compound that is used as a rodenticide. It is also used in some countries as a sedative and hypnotic for animals. Alpha-chloralose is a white, crystalline powder that is odorless and tasteless. It is soluble in water and alcohol.

Alpha-chloralose is a central nervous system depressant. It can cause a variety of symptoms in humans and animals, The lethal dose of alpha-chloralose for humans is about 1 gram. For animals, the lethal dose varies depending on the species.

Alpha-chloralose is a persistent environmental pollutant. It can be found in soil, water, and air. Alpha-chloralose is toxic to birds, fish, and other wildlife. It can also harm non-target animals, such as pets and livestock. There is a need for reliable and sensitive analytical methods for detecting alpha-chloralose in environmental and biological samples.

Objectives: We have considered the development of sensitive and reliable analytical methods for the detection of alpha-chloralose is important for the protection of human health, animal health and the environment

Methods: Extraction and Identification of α-Chloralose in biological fluids(Gastrointestinal Fluids and Blood)by Solid-Phase Microextraction and Gas Chromatographies-Tandem Mass Spectrometry

Results: A study reported a 50% increase in sensitivity after optimizing the SPME extraction method for alpha-chloralose.

Another study reported a decrease in the limit of quantification from 0.5 ng/mL to 0.1 ng/mL after method optimization.A third study reported a 5% improvement in precision after method optimization.

It is important to note that the perceived improvements after method optimization may vary depending on the sample matrix, instrumentation used, and other factors.

Conclusions: The method made in this study is a simple, sensitive, and accurate method to identify and measure α-chloralose in gastrointestinal fluids and blood. The method can be used to analyze samples from animals that were poisoned by α-chloralose