Extraction and Determination of Trace Copper (II) Using Octadecyl Silica Membrane Disks Modified 1-(2-Pyridyl Azo) 2-Naphtol(Pan) in Water Samples and Paraffin-Embedded Tissues from Liver Loggerhead Turtles Specimens by FAAS

Abstract

A simple and reproducible method for the rapid extraction and determination of trace amounts of
copper(II) ions using octadecyl-bonded silica membrane disks modified by 1-(2-Pyridyl Azo)2-Naphtol(PAN)
and Atomic Absorption Spectrometry was presented. The method was based on complex formation on the
surface of the ENVI-18 DISKTM disks followed by stripping of the retained species by minimum amounts of
appropriate organic solvents. The elution was efficient and quantitative. The effect of potential interfering ions,
pH, ligand amount, stripping solvent, and sample flow rate were also investigated. Under the optimal
experimental conditions, the break-through volume was found to about 1000mL providing a preconcentration
factor of 500. In the present study, we reported the application of preconcentration techniques still continues
increasingly for trace metal determinations by flame atomic absorption spectrometry (FAAS) for quantification
of Cu in Formalin-fixed paraffin-embedded (FFPE) tissues from Liver loggerhead turtles. The maximum
capacity of the disks was found to be 389± 4 μg for Cu2+.The limit of detection of the proposed method was 5ng
per 1000mL.The method was applied to the extraction and recovery of copper in different water samples.