HPLC method for simultaneous estimation of Remogliflozin and Teneligliptin

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Remogliflozin and Teneligliptin in tablet dosage form. Chromatogram was run through Std Azilent 150(4.6 x 150mm, 5µm). Mobile phase containing Acetonitrile: Formic acid taken in the ratio 65:35 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method Phosphate buffer and ph is adjusted to 5.4 by adding 0.1% Formic acid. Temperature was maintained at 30°C. Optimized wavelength selected was 228 nm. Retention time of Remogliflozin and Teneligliptin were found to be 2.189 min and 2.824 min. %Recovery was obtained as 99.89% and 100.27% for Remogliflozin and Teneligliptin respectively. LOD, LOQ values obtained from regression equations of Remogliflozin and Teneligliptin were 0.66, 2.00 and 0.53, 1.61 respectively. Regression equation of Remogliflozin is y = 11909x +42491. And y = 11725x + 1485of Teneligliptin. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.