Synthesis, Characterization, and Insilico Nanochitosan of Pupa Black Soldier Fly (Hermetia Illucens) As Bone Graft Material for Bone Remodeling Post Tooth Extraction

Abstract

Objectives: This research aims to make nanochitosan BSF pupae through ionic gelation and size reduction with a magnetic stirrer and to determine the bonding of chitosan compounds and their derivatives through the insilico method for forming alveolar bone.

Methods: BSF chitosan is made through demineralization, deproteination, depigmentation, and deacetylation, then formulated into nanochitosan using the ionic gelation method. The reduction in particle size uses a magnetic stirrer by adding Na-TPP 0.1%. The bonding of chitosan compounds and their derivatives uses the insilico method through the Autodock Vina program, which is integrated into the PyRx application version 0.8. Docking results of chitosan compounds using Biovia Discovery Studio V21.1.0.20298 software.

Results: The Deacetylation of chitosan reached 80%, indicating the purity of chitosan and according to SNI No.7949-2013. The nanochitosan formed has an average size of 204.9 nm, is reasonably uniform, relatively stable, and has a spherical shape resembling a ball. The Glycol Chitosan and Aminoethyl-Chitosan compounds have many hydrogen bonds. The lowest binding affinity value is -9.8 kcal/mol in Glycol Chitosan and has identical amino acid residues as the comparison ligand in Aminoethyl-Chitosan.

Conclusions: Chitosan extracted from pupa BSF (Hermetia illucens) has fulfilled SNI No.7949-2013 including water content test, ash content and degrees of deacetylation with a yield of 13.2%. The degree value of the deacetylation of chitosan used to make nanocitosan is 80%, showing that the resulting chitosan is pure chitosan. The characterization of nanoparticles is formed an average size of 204.9 ± 48.7 nm, quite uniform and relatively stable.